Scooby and Shaggy meet up with their biker buddy, Axl Rose, on a tour of famous roadside diners along iconic Route 66. However, they soon find themselves embroiled in a crazy spooky mystery involving three mud men.

Scooby Doo takes the gang to meet his good friend Lucy Liu at her art gallery in Queens: when one of her large humanoid modern sculptures comes alive and attacks them, the gang and Lucy have a real mystery on their hands.