Batman is dead, and a powder keg has ignited Gotham City without the Dark Knight to protect it. In the wake of Bruce Wayne's murder, his adopted son Turner Haye is framed for killing the Caped Crusader, along with the children of some of Batman's enemies: Duela Doe, aka The Joker's Daughter, an unpredictable fighter and skilled thief who was born in Arkham Asylum and abandoned by her father, Harper Row, a streetwise and acerbic engineer who can fix anything, and her brother Cullen Row, a clever transgender teen who is tired of being polite and agreeable. With the charismatic and hard-charging District Attorney Harvey Dent and the GCPD hot on their trail, Turner will rely on allies including his best friend and formidable coder Stephanie Brown, and unlikely Batman sidekick Carrie Kelley. But our Knights will soon learn there is a larger, more nefarious force at work within Gotham City. This team of mismatched fugitives must band together to become its next generation of saviors known as the "Gotham Knights."

After their leader is taken down by Carrie, the Mutant Gang retaliates against Gotham by taking hostages at the annual Founder's Gala, forcing Turner, Duela and Harper to come out of hiding to save the people of Gotham. Elsewhere, Cullen infiltrates the GCPD after the team uncovers a possible connection between the Court of Owls and the death of another Wayne family member. Lastly, Harvey makes a big decision about his future.