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Dean must deal with the aftermath of the fight between Sam and Jake and pays a steep price for his involvement. The Demon and his spirits threaten to overtake the hunters until they receive help from an unexpected ally.
Sam is abducted by the Yellow-Eyed Demon and finds himself in a ghost town with other children who have special abilities. They learn the Demon has brought them together to initiate his endgame: an all-out war against the human race. In an effort to encourage Sam to fight, the Demon shows him what really happened to one of his parents. Dean enlists Bobby's help to find Sam before the battle begins, but he arrives too late.
The season follows Sam and Dean Winchester as they deal with their father's death and continue to hunt down the yellow-eyed demon known as Azazel, who caused the fire that led to the deaths of their mother over 20 years ago and later, Sam's girlfriend, Jessica Moore. They have assistance not only from their friend Bobby, but from new allies Ellen Harvelle, her daughter Jo, and Ash. Part of Azazel's master plan is eventually revealed as he gathers Sam and others like him, leading to Sam's death. Dean makes a deal with a crossroads demon to resurrect Sam in exchange for his soul, which will be collected in one year. In a final confrontation, in which Dean kills Azazel with The Colt using the last of the original bullets, a gate is opened, releasing hundreds of demons from Hell.