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Sam, Dean and John were left for dead after the Demon plowed a semi into the Impala. Now, one of the Winchesters hovers between life and death as the surviving family must face an old and powerful adversary.
Sam and Dean listen to a message on John's cell phone from a woman named Ellen and decide to track her down. They are shocked to discover Ellen runs a road house that also serves as a gathering place for hunters like themselves. Meanwhile, at a small town carnival, a clown is persuading children to let them into their home so he can murder their parents.
The season follows Sam and Dean Winchester as they deal with their father's death and continue to hunt down the yellow-eyed demon known as Azazel, who caused the fire that led to the deaths of their mother over 20 years ago and later, Sam's girlfriend, Jessica Moore. They have assistance not only from their friend Bobby, but from new allies Ellen Harvelle, her daughter Jo, and Ash. Part of Azazel's master plan is eventually revealed as he gathers Sam and others like him, leading to Sam's death. Dean makes a deal with a crossroads demon to resurrect Sam in exchange for his soul, which will be collected in one year. In a final confrontation, in which Dean kills Azazel with The Colt using the last of the original bullets, a gate is opened, releasing hundreds of demons from Hell.