Gil Grissom is the supervisor of the graveyard shift of Las Vegas' elite crime scene investigators. Holly Gribbs, fresh out of the academy, joins the team. Jim Brass is head of the unit and is trying to scare Gribbs from CSI. Catherine Willows, mother of a young daughter, is the number two CSI on this shift. Warrick Brown and Nick Stokes are competing to solve their 100th case to earn a promotion to CSI 3. Grissom investigates a murder staged to look like a suicide with precious little evidence. Warrick and Catherine take the case of a drunk who breaks into the house where he had been staying and is shot to death. Nick talks to a man who picked up a woman who drugged and robbed him.

Grissom, Nick, and Sara search for a kidnapped woman and find more than they expected. Catherine and Warrick investigate the hit and run death of a little girl.