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Beginning at the river's source in Gloucestershire, Tony meets a professional crayfish catcher, investigates the water's cleanliness, and learns the uses the Home Guard put the river to in WWII.
The actor and presenter follows the river as it winds its way through the Thames valley, stopping off at Henley-on-Thames, and then on towards the palace at Hampton Court. In the village of Clifton Hampden, he tries his hand at sailing a skiff, and later visits Thames Water's most advanced sewage plant. In Henley-on-Thames, Tony attempts to cox a crew from the elite Leander Rowing Club, Britain's most successful sporting team who boast 124 Olympic medals, and at Hampton Court Palace, he boards the gold-encrusted Glorianna, the Queen's Royal Barge that led 1,000 boats down the Thames during Her Majesty's diamond jubilee.