In the second episode, Tony explores the river's role as a vital trade artery connecting the country to the outside world, going behind the scenes at London Gateway, the newest and largest container port on the Thames. Later, he visits Whitstable, famous for its oysters that come from the Thames estuary.

The presenter watches a tunnelling machine being transported up the river into central London, and discovers plans to build a new tunnel under the Thames to take pressure off the crossing at Dartford. He also makes a late-night visit to New Covent Garden Market, and meets a Paralympian in training at Marlow Rowing Club.