Kate Humble and Ben Fogle return to Longleat Safari Park to join the keepers and animals as the park springs into action for another season. The keepers get a shock when three highly endangered cheetah cubs are born, the first in the park's history. There is also a catch-up with Anne, the rescued circus elephant, and the animal osteopath who is helping to manage her arthritis. Meanwhile, on Penguin Island, a killer disease strikes and hand-reared birds begin to die in the arms of the people who raised them. Can a cure be found before it is too late?

Keepers are locked in a battle of wills with Dave the Canadian timber wolf, who keeps attempting to explore areas outside of his enclosure. Having worked at the park for 40 years, one keeper might have finally got the two grumpy hippos just where he wants them. Meanwhile, Jean discovers just how powerful an elephant sneeze really is. And as the sun sets on the lion paddock, keepers are rigging special cameras to try to discover what goes on after dark.