As a heatwave scorches the Everglades, Troy and Tes intercept pythons charging toward the rivers. Zak and Aaron venture far west to a remote canal. Ashley and Kaylyn gamble on an all-night hunt. Dusty scavenges for his next meal.

In the final hours of python season, hunters launch a full assault to win the battle against the vicious invaders. With the clock ticking to save the Everglades, teams race back to old honey holes to track down the most elusive beasts, while Dusty bids farewell to his longtime partner.