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A mad art connoisseur seizes control of the Seaview and plans to pirate Leonardo da Vinci's Mona Lisa from a French Ship bound for the World's Fair.
After returning from a mission on deep space, a lifelike robot is brought aboard the Seaview, but the crew soon discovers that the robot has been mysteriously reprogrammed to wreak havoc.
The Seaview is selected to be converted to a totally automatic ship controlled by a central computer. The mission goes well till Capt. Crane, the only person aboard the sub, realizes he may not be as alone as he thought.