After locating and retrieving the Amulet of Juatchdoon, adventuring archeologist duo Ohio (Phineas) Flynn and Rhode Island (Ferb) Fletcher set off to South America to help Damsel in Distress Isabella find the Lost Temple of Juatchadoon and her missing mother. However, Dr. Doofenshmirtz has other plans for the amulet - to awaken the evil corn colossus to give him the power to destroy the world.

Thinking it would be cool to be part of an ant society, Phineas and Ferb create and infiltrate the Antius Maximus, a massive ant farm filled with giant ants. Suspecting that the boys are up to something, Candace accidentally drenches herself in the ant pheromones and follows them inside. Meanwhile, Doofenshmirtz feels tired and lethargic after eating turkey and decides to create a Turkey-Inator that will create a sleeping epidemic in Danville by turning things into giant irresistible turkeys.