American teenage witch Sabrina and her food-obsessed magical talking cat and mouse travel to Rome, the last whereabout of her 16th-century aunt Sophia, who was banished after breaking the rule not to divulge her powers to a non-witch; her mission is to find out how Sophia can be freed by opening her golden locket.

Trouble brews between best friends when Valerie becomes eager to try out for cheerleading, even though Sabrina can't stand the squad's pom-pompous participants.