A young zoologist named Yamato Kazakiri is transported to Zyuland, a mysterious land inhabited by humans with animal-like features. When he returns to Earth accompanied by four Zyumans, Sela, Leo, Tusk and Amu, the planet is under attack by the Dethgaliens, who chose it as the venue for their 100th Blood Game, and with the power of the Champion's Proofs, artifacts that serve as the link between both realms, they transform for the first time in the Zyuohgers to repel the enemy attack.

The Zyumans cannot return to Zyuland until they find the missing sixth Champion's Proof. Amu thinks she has found it and takes Yamato along with her, but she seems more interested in experiencing human culture. Their plans are waylaid when the Dethgalien Player Bowguns attacks various cars and trucks, so the Zyuohgers face off, but find that his attacks are too fast to be seen, until Yamato seems to gain a new power.