Born into a multigenerational law enforcement family, Special Agent Maggie Bell (Missy Peregrym) commits deeply to the people she works with as well as those she protects. Her partner is Special Agent Omar Adom "OA" Zidan (Zeeko Zaki), a West Point graduate via Bushwick who spent two years undercover for the DEA before being cherry-picked by the FBI.

Overseeing them is Special Agent in Charge Dana Mosier (Sela Ward), who operates under intense pressure 24/7/365 and has undeniable command authority. The team also includes Assistant Special Agent in Charge Jubal Valentine (Jeremy Sisto), the nerve center of the office whose ability to easily relate to and engage with both superiors and subordinates makes him a master motivator. Kristen Chazal (Ebonée Noel) is the team's most valued resource, a brilliant analyst recruited straight out of university who can piece together the big picture faster than anyone. These first-class agents tenaciously investigate cases of tremendous magnitude, including terrorism, organized crime and counterintelligence.