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Virginia and Dr. Masters move on to filming involuntary reactions during the study, with Jane as usual being the first subject. Ethan reveals information about his heritage that threatens their relationship.
Virginia resumes pursuit of her undergraduate degree. Masters takes Virginia's suggestion and enlists the aid of a technician to construct a working camera to film the intimate workings during sexual intimacy.
A former study participant comes forward with news of her pregnancy. Ethan learns he has been effectively fired, but not for the reason he thought.
Former singer and single mother Virginia Johnson becomes the assistant to renowned OBGYN William Masters. They embark on their pioneering study in human sexuality while trying to navigate their personal feelings for each other.