The Cajun Navy is back to fight the war against pythons invading the Everglades. King of the Swamp Troy Landry, along with his son Chase and snake expert Bill Booth, track serpents into a breeding ground known as Deer Haven. Meanwhile, veteran swamper Bruce Mitchell and invasive species hunter, Zak Catchem, take on the legendary Skunk Ape Alley, an area known for strange encounters and massive pythons.

The hunters venture into the Everglades Triangle, an area known for mysterious disappearances and swarms of snakes; Troy, Chase and Bill use a drone to search the swamp from the air, while Bruce and Zak track snakes with a handheld thermal device.