Troy Landry teaches long-time partner Pickle Wheat the ins and outs of python hunting; veteran snake hunter Bill Booth and Florida hunter Tes Lee must learn to work as a team if they hope to put a dent in the growing python population.

Troy and Pickle hunt a giant python known as the Night Serpent. Tess convinces Bill to try out new hunting grounds, but Bill is not sure it's a good idea. Zak and Aaron explore a notorious corner of the Everglades, and Dusty Crum has an idea to lighten his load.