Like the saying goes, "If you stay ready, you never have to get ready"; and that is a code Tampa's first responders live by. Robb and Bethany rush to the scene where a man is shot by the police, while Tony and Dave have to think quickly to treat a stabbing victim, and Stokes and Mayville rush to put out a grocery store fire.

Pierre and Jerry are backed up by Air Service when they go on a high-speed car chase through the streets of Tampa. Rush and Benny have to restrain a combative patient, while Stokes and Doug transport a victim of sexual assault in Ybor. Robb and Bethany witness a heartbreaking scene when a mother must decide whether or not to send her son to jail in order to save his life.

Chaos erupts when shots are fired and a teenager is wounded while Will and Dimitri are on patrol at a high school football game. Meanwhile, Mayville and Stokes are on the scene when multiple bomb threats are made in Ybor City. And the ROC Squad are on the move when they chase down a teenager on a joyride with his parents' car.

It's Halloween night in Tampa and the cases get wild. Tony and Dave treat a woman who was shot in the chest with a BB gun and is bleeding uncontrollably. The ROC Squad responds to a call from a man who was shot, and Will and Dimitri go into detective mode to help track down the assailants. Later on, Air Control guides Robb and Bethany to track down a fleeing suspect who thinks he can hide in a trash dumpster.

Tampa first responders have a chaotic night treating seizure and overdose patients, while the ROC Squad Task Force tracks down suspected armed robbers in a high-speed chase.

Officers Robb and Bethany are helped by the K9 Unit to corral a suspect who takes off running after a car chase. Fire Rescue's Rush and Rutledge struggle to convince a man with heart problems to go to the hospital. Mayville and Doug are called in when a car careens off a bridge–and the driver can't be found.

Mark and Austin find themselves conflicted when a man stabbed multiple times angrily refuses treatment. Jerry and Pierre engage in a car chase that results in a foot chase when an armed suspect bails from the vehicle into a parking lot.

Officers Roni and Cooper engage in a foot chase following a stabbing at a nightclub; Fire Rescue's Doug and Stokes try to save the life of a man shot in the throat; the hunt for a carjacker leads Will and Dimitri to the roof of an apartment complex.

Family bonds and family struggles take center stage when a man approaches Robb and Bethany and asks them to help find his missing daughter whom he fears is dead. The ROC Squad set up a high-profile sting to capture a group of thieves preying upon Tampa. Rush and Rutledge try to save a puppy from a raging apartment complex fire.

In the premiere Tampa episode, we meet the brave members of the police and fire rescue departments that risk their lives every night patrolling the city to protect its residents. Police officers Robb and Bethany find themselves in an armed standoff with a suspect who has been holed up in an apartment complex. And fire rescue team Mayville and Stokes must put out a trailer fire that threatens to incinerate the rest of the residential park.