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Both Bill and Virginia have unexpected and unwelcome visitors at home. With the study back in the hospital, new participants are recruited. Ethan struggles to forget his feelings for Virginia.
A woman pregnant with quadruplets comes to the hospital and Dr. Haas lobbies for the case. A female gynecologist joins the staff. Both Dr. Masters and his wife learn a secret and both get what they want.
William and Libby experience personal tragedy. Masters decides to pair Austin with a stranger. Virginia struggles with her parenting due to her long hours. Ethan's relationship with Vivian takes the next step, whether he likes it or not.
Former singer and single mother Virginia Johnson becomes the assistant to renowned OBGYN William Masters. They embark on their pioneering study in human sexuality while trying to navigate their personal feelings for each other.