Oti and Motsi Mabuse head on a journey across their homeland of South Africa to explore their extraordinary family history. The sisters have tried to explore their history before, but with records for black South Africans almost nonexistent, going on their DNA journey is the last roll of the dice to discover their roots.

In episode 3, Line of Duty stars Neil Morrissey and Adrian Dunbar met on their audition day for Guildhall drama school in London. A meeting that sparked a 42 year long friendship. Now using DNA and genealogy they are on a journey that will take them across the UK and Ireland, reconnecting with lost cousins and unearthing long held family secrets. 

Anarchic comedian Johnny Vegas and broadcaster Alex Brooker embark on a road trip to explore their family histories. Alex knows nothing about his family history, and is blown away to find a relative who met Jack the Ripper. Meanwhile, Johnny is aware that generations of his family have worked in St Helens, and is fascinated to learn of the ancestor who moved his family northwards through the booming industry of glassblowing.

Acclaimed actor and Downton Abbey star Hugh Bonneville, and one of the UK's successful comedians John Bishop, make for an unlikely friendship. However, a chance meeting in a Covid vaccine centre has made them mates and now they're about to cement that friendship on a road trip to discover their family history - but what they both don't know is that they have a deeper connection to each other through their ancestors.