Dudley Puppy works as a spy for an organization called T.U.F.F. (short for Turbo Undercover Fighting Force). His partner is a cat named Kitty Katswell. Other helpers are The Chief and Keswick. The series takes place in a fictional city called Petropolis, which is populated by anthropomorphic animals. As a member of T.U.F.F., Dudley Puppy helps Kitty Katswell protect Petropolis from various villains like Verminious Snaptrap, the Chameleon, and Birdbrain.